| Autor: |
Hemati, Maral, Soosanabadi, Mohsen, Ghorashi, Tahereh, Ghaffari, Hadi, Vahedi, Azadeh, Sabbaghian, Elaheh, Rasouli Nejad, Zahra, Salati, Amir, Danaei, Navid, Kokhaei, Parviz |
| Předmět: |
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| Zdroj: |
Journal of Cellular Physiology; Jul2021, Vol. 236 Issue 7, p4966-4972, 7p |
| Abstrakt: |
The rapid spread of coronavirus disease 2019 (COVID‐19), a disease caused by severe acute respiratory syndrome coronavirus 2, poses a huge demand for immediate diagnosis. Real‐time reverse transcriptase‐polymerase chain reaction (rRT‐PCR) of nasopharyngeal (NP) and oropharyngeal (OP) swabs have been used to confirm the clinical diagnosis. To avoid the risk of viral‐exposure of laboratory workers, thermal inactivation is currently recommended but has unknown effects on the accuracy of the rRT‐PCR results. Thirty‐six NP/OP specimens were collected from COVID‐19 patients and subjected to thermal inactivation (60°C for 30 min) or the RNA extraction processes to activate the form. Here, our data showed that the concentration of extracted‐RNA increases upon thermal inactivation compared to the active form (p =.028). Significantly higher levels of RNA copy number were obtained in inactivated compared to the active samples for both N and ORF1ab genes (p =.009, p =.032, respectively). Thermal inactivation elevated concentration and copy number of extracted‐RNA, possibly through viral‐capsid degradation and/or nucleoprotein denaturation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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