| Autor: |
SI Neng-wu, LI Jun, WU Yu-hua, WU Gang, ZHANG Li |
| Předmět: |
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| Zdroj: |
Chinese Journal of Oil Crop Sciences; 2021, Vol. 43 Issue 1, p40-50, 11p |
| Abstrakt: |
Labeling of genetically modified organisms (GMOs) is an important part of safety management of GMOs. Quantitative detection technology provides important technical support for effective implementation of the GM labeling system. Digital PCR does not rely on standard curves and reference materials when performing quantitative detection of nucleic acid molecules, it could directly calculate copy number concentration of the detection target in sample. Its application in accurate quantification of nucleic acids is becoming more and more extensive. In the quantitative detection of GMOs, digital PCR could be used directly to calculate content of exogenous gene in template. By comparing of digital PCR and real-time fluorescent quantitative PCR in GMOs detection, factors affecting digital PCR detection results were elaborated, and the possibility of digital PCR results traceable to SI units was discussed. This study was expected to provide a reference for application of digital PCR in quantitative detection of GMOs and precise quantification of nucleic acids in several fields. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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