| Autor: |
Pennacchiotti, Gina, Valdés-Gutiérrez, Fabio, González-Arriagada, Wilfredo Alejandro, Montes, Héctor Federico, Parra, Judith Maria Roxana, Guida, Valeria Andrea, Gómez, Silvina Esther, Guerrero-Gimenez, Martin Eduardo, Fernandez-Muñoz, Juan Manuel, Zoppino, Felipe Carlos Martin, Carón, Rubén Walter, Ezquer, Marcelo Eduardo, Fernández-Ramires, Ricardo, Bruna, Flavia Alejandra |
| Předmět: |
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| Zdroj: |
Scientific Reports; 3/25/2021, Vol. 11 Issue 1, p1-11, 11p |
| Abstrakt: |
The oral squamous cell carcinoma (OSCC), which has a high morbidity rate, affects patients worldwide. Changes in SPINK7 in precancerous lesions could promote oncogenesis. Our aim was to evaluate SPINK7 as a potential molecular biomarker which predicts OSCC stages, compared to: HER2, TP53, RB1, NFKB and CYP4B1. This study used oral biopsies from three patient groups: dysplasia (n = 33), less invasive (n = 28) and highly invasive OSCC (n = 18). The control group consisted of clinically suspicious cases later to be confirmed as normal mucosa (n = 20). Gene levels of SPINK7, P53, RB, NFKB and CYP4B1 were quantified by qPCR. SPINK7 levels were correlated with a cohort of 330 patients from the TCGA. Also, SPINK7, HER2, TP53, and RB1, were evaluated by immunohistofluorescence. One-way Kruskal–Wallis test and Dunn's post-hoc with a p < 0.05 significance was used to analyze data. In OSCC, the SPINK7 expression had down regulated while P53, RB, NFKB and CYP4B1 had up regulated (p < 0.001). SPINK7 had also diminished in TCGA patients (p = 2.10e-6). In less invasive OSCC, SPINK7 and HER2 proteins had decreased while TP53 and RB1 had increased with respect to the other groups (p < 0.05). The changes of SPINK7 accompanied by HER2, P53 and RB1 can be used to classify the molecular stage of OSCC lesions allowing a diagnosis at molecular and histopathological levels. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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