| Abstrakt: |
The aim of this study was to evaluate the 28 days repeated dose oral toxicity or subacute oral toxicity study of hydroalcoholic extract of Helicteres isora (HAEHI) dried fruits in male and female experimental wistar rats. This toxicity study was carried out at 200mg/kg, 500mg/kg and 1000mg/kg of doses of hydroalcoholic extract of Helicteres isora dried fruits. During the study period of 28 days all the animals were observed weekly for changes in body weight, food consumption, water consumption, behavior changes, any sign of morbidity and mortality. After 28 days of study period, all the animals were humanly sacrificed by using cervical dislocation technique and were examined for changes in their relative organ weight, biochemical and hematological parameters and also observed for histopathological changes. The results of the present study indicated that there is increase in food consumption as well as elevated body weight at 1000mg/kg doses in both male and female wistar rats. The results of biochemical tests and hematological tests show significant changes in some parameters at higher dose of HAEHI. In the histopathology, animals treated with extract at 1000mg/kg body weight showed minimal tubular degeneration in kidney of both male and female wistar rats; however, liver, heart and lungs did not show any toxicity at any of the doses when compared with control group animals. From results, our study concludes that repeated administration of hydroalcoholic extract of H. isora dried fruits for 28 days increases the food consumption along with body weight and indicates its role as dietary supplement. The hydroalcoholic extract of Helicteres isora dried fruits was found to be safe in 28 days repeated dose toxicity study. While at higher doses it shows minor significant alteration in histopathology, biochemical and hematological parameters. [ABSTRACT FROM AUTHOR] |
