| Autor: |
Stavinoha, Melissa A., RaySpellicy, Joseph W., Essop, M. Faadiel, Graveleau, Christophe, Abel, E. Dale, Hart-Sailors, Mary L., Mersmann, Harry J., Bray, Molly S., Young, Martin E. |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Endocrinology & Metabolism; Nov2004, Vol. 287, pE888-E895, 8p, 3 Charts, 15 Graphs |
| Abstrakt: |
The physiological role of mitochondrial thioesterase 1 (MTE1) is unknown. It was proposed that MTE1 promotes fatty acid (FA) oxidation (FAO) by acting in concert with uncoupling protein (UCP)3. We previously showed that ucp3 is a peroxisome proliferator-activated receptor-α (PPARα)-regulated gene, allowing induction when FA availability increases. On the assumption that UCP3 and MTE1 act in partnership to increase FAO, we hypothesized that intel is also a PPARαregulated gene in cardiac and skeletal muscle. Using real-time RTPCR, we characterized mte1 gene expression in rat heart and soleus muscles. Messenger RNA encoding for mte1 was 3.2-fold higher in heart than in soleus muscle. Cardiac mte1 mRNA exhibited modest diurnal variation, with 1.4-fold higher levels during dark phase. In contrast, skeletal muscle mte1 mRNA remained relatively constant over the course of the day. High-fat feeding, fasting, and streptozotocin-induced diabetes, interventions that increase FA availability, muscle PPARα activity, and muscle FAO rates, increased mte1 mRNA in heart and soleus muscle. Conversely, pressure overload and hypoxia, interventions that decrease cardiac PPARα activity and FAO rates, repressed cardiac mte1 expression. Specific activation of PPARα in vivo through WY-14643 administration rapidly induced mte1 mRNA in cardiac and skeletal muscle. WY-14643 also induced mte1 mRNA in isolated adult rat cardiomyocytes dose dependently. Expression of intel was markedly lower in hearts and soleus muscles isolated from PPARα-null mice. Alterations in cardiac and skeletal muscle ucp3 expression mirrored that of mte1 in all models investigated. In conclusion, mte1, like ucp3, is a PPARα-regulated gene in cardiac and skeletal muscle. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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