| Autor: |
Schneider, Cosette G., Taylor, Justin A., Sibilo, Michael Q., Miura, Kazutoyo, Mallory, Katherine L., Mann, Christopher, Karch, Christopher, Beck, Zoltan, Matyas, Gary R., Long, Carole A., Bergmann-Leitner, Elke, Burkhard, Peter, Angov, Evelina, Tripp, Ralph A. |
| Předmět: |
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| Zdroj: |
Vaccines; Feb2021, Vol. 9 Issue 2, p103, 1p |
| Abstrakt: |
Self-assembling protein nanoparticles (SAPN) serve as a repetitive antigen delivery platform with high-density epitope display; however, antigen characteristics such as size and epitope presentation can influence the immunogenicity of the assembled particle and are aspects to consider for a rationally designed effective vaccine. Here, we characterize the folding and immunogenicity of heterogeneous antigen display by integrating (a) dual-stage antigen SAPN presenting the P. falciparum (Pf) merozoite surface protein 1 subunit, PfMSP119, and Pf cell-traversal protein for ookinetes and sporozoites, PfCelTOS, in addition to (b) a homogenous antigen SAPN displaying two copies of PfCelTOS. Mice and rabbits were utilized to evaluate antigen-specific humoral and cellular induction as well as functional antibodies via growth inhibition of the blood-stage parasite. We demonstrate that antigen orientation and folding influence the elicited immune response, and when appropriately designed, SAPN can serve as an adaptable platform for an effective multi-antigen display. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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