| Autor: |
De Bie, J., Smits, A., Marei, W. F. A., Leroy, J. L. M. R. |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility & Development; 2021, Vol. 33 Issue 4, p291-304, 14p |
| Abstrakt: |
Trials to improve oocyte developmental competence under metabolic stress by using antioxidants may start before or after oocyte maturation. In the present conceptual study, we aimed to identify the most efficient timing of antioxidant application in relation to a metabolic insult using a bovine in vitro embryo production model. Pathophysiological concentrations of palmitic acid (PA) were used to induce metabolic stress during oocyte maturation or embryo development. Trolox (TR; antioxidant) treatment prior to, during or after the PA insult was tested to evaluate the protective, neutralising and rescuing capacity of TR respectively. Changes in embryo developmental competence, mitochondrial activity, reactive oxygen species (ROS) concentrations, blastocyst cell allocation and apoptosis and cell stress-related gene expression were monitored. The improvement in developmental capacity was most obvious when oocytes were preloaded with TR before the PA insult. This protective effect could be explained by the observed combination of increased mitochondrial activity with reduced ROS production. This resulted in blastocysts with normal cell counts and apoptosis, as well as increased nuclear factor erythroid 2-related factor 2 (NRF2) expression (a marker for redox regulatory processes) and normalised the expression of the mitochondrial transcription factor A (TFAM), a marker of mitochondrial biogenesis. These results indicate that 'pretreatment' of oocytes with antioxidants produces embryos that seem to be more resilient to a metabolic stress insult. Suboptimal nutrition and metabolic health can increase oxidative stress in oocytes and embryos, thereby affecting fertility. The strategic use of antioxidants protects oocytes against metabolic stress insults during embryo development. These findings highlight the importance of the timing of antioxidant treatment with the aim of protecting and safeguarding oocyte and embryo quality, and improving fertility. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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