Morphological and physiological responses of tara (Caesalpinia spinosa (Mol.) O. Kuntz) microshoots to ventilation and sucrose treatments.

Autor: Núñez-Ramos, Jenny Elizabeth, Quiala, Elisa, Posada, Laisyn, Mestanza, Saúl, Sarmiento, Leidy, Daniels, Dion, Arroyo, Carlos R., Naranjo, Blanca, Vizuete, Karla, Noceda, Carlos, Gómez-Kosky, Rafael
Zdroj: In Vitro Cellular & Developmental Biology Plant; Feb2021, Vol. 57 Issue 1, p1-14, 14p
Abstrakt: One of the main problems of in vitro plant tissue culture is the poor ventilation in the culture vessels. This leads to morphological and physiological anomalies in regenerated in vitro plants, a crucial issue in woody species, such as Caesalpinia spinosa (Mol.) O. Kuntz, which is a legominous tree or thorny shrub of the Andes, adequate photoautotrophic and photomixotrophic in vitro rooting systems could eliminate these problems. Therefore, the purpose of the present study was to evaluate photoautotrophic and photomixotrophic treatments to optimize the in vitro rooting of C. spinosa microshoots. Micropropagated shoots were cultured in vessels sealed with a polypropylene lid with 0, 1, or 3 ventilation holes covered with a 0.45-μm adhesive polypropylene microfilter. The vessels contained MS salts with or without 30 g L−1 sucrose or, alternatively, a porous substrate (PRO-MIX®), which facilitated rooting and produced greater fresh and dry weights, a higher concentration of photosynthetic pigments and phenolic compounds, and more developed leaves. The optimal performance and higher survival was obtained by using PRO-MIX® with three filters for increased ventilation. For treatments in agar, only the one with sucrose and highly ventilated (three filters) reached a performance approaching that with PRO-MIX®. To date, the presented results have not been reported in scientific literature for C. spinosa. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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