Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe.

Autor: Hatai, Joydev, Prasad, Pragati Kishore, Lahav-Mankovski, Naama, Oppenheimer-Low, Noa, Unger, Tamar, Sirkis, Yael Fridmann, Dadosh, Tali, Motiei, Leila, Margulies, David
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Zdroj: Chemical Communications; 2/21/2021, Vol. 57 Issue 15, p1875-1878, 4p
Abstrakt: Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in live Escherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index