| Abstrakt: |
Vibrissa dermal papilla cells have been successfully grown and serially cultured and their early behaviour compared with cultured feather dermal papillae. Initially the vibrissa cells demonstrated singular characteristics, including poor spreading ability, before eventually forming a fibroblast-like population of cells. Comparatively few studies have exploited the adult hair follicle as a source of cell culture material. While cultures have been established by complete disruption of lower hair bulbs (Frater, 1975) and from mildly trypsinized explants (Uzuke, Takeshita & Morikawa, 1975), both methods produce a mixed population of cells, whose exact intrafollicular site of origin is unknown. The large size of the vibrissa follicle makes it amenable to microsurgical manipulation of discrete follicular components, and in vivo studies have shown the dermal papilla to be necessary for both the induction and maintenance of hair growth (Oliver, 1966, 1967). This study investigated the possibility of growing a population of dermal papilla cells in culture and the observations constitute the first description of these important and highly specialized cells in vitro. [ABSTRACT FROM AUTHOR] |
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