| Autor: |
Horie, Yoshifumi, Kanazawa, Nobuhiko, Takahashi, Chiho, Tatarazako, Norihisa, Iguchi, Taisen |
| Předmět: |
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| Zdroj: |
Journal of Applied Toxicology; Mar2021, Vol. 41 Issue 3, p399-409, 11p |
| Abstrakt: |
The branched isomer mixture 4‐nonylphenol (4‐NP) has been used worldwide as a surfactant, and can have endocrine‐disrupting effects on aquatic organisms. For instance, 4‐NP induces the formation of testis‐ova (i.e., testicular and ovarian tissue in the same gonad) or male to female sex reversal of various teleost fishes. Recently, our group revealed that altered gsdf gene expression is associated with disruption of gonadal differentiation in Japanese medaka (Oryzias latipes) embryos exposed to methyltestosterone or bisphenol A, suggesting that gsdf might be useful as a biomarker for predicting the impact of endocrine‐disrupting chemicals (EDCs) on gonadal differentiation. Here, we used 4‐NP to examine further whether gsdf expression at the embryo stage is useful for predicting EDC impact on gonadal sex differentiation. When fertilized medaka eggs were exposed to 32 or 100 μg/L 4‐NP, testis‐ova in genetic males and sex reversal from genetic male to phenotypic female were observed. At stage 38 (just before hatching), 4‐NP exposure at 1‐100 μg/L did not affect gsdf expression in XX embryos compared with the nontreated control; however, in XY embryos, the gsdf expression in the 100 μg/L‐exposed group was significantly lower than that in the controls. The 4‐NP concentration at which gsdf expression was suppressed was equal to that at which testis‐ova and sex reversal were induced. These results indicate that expression of the gsdf gene at the embryonic stage in medaka is a useful biomarker for predicting the impact of EDCs on sexual differentiation. Recently, we revealed that the gsdf gene may be a useful biomarker for predicting endocrine‐disrupting chemicals (EDCs). We found that exposure to 4‐nonylphenol (4‐NP) induced testis‐ova or sex reversal and suppressed gsdf expression in Oryzias latipes. The concentration at which gsdf expression was suppressed by 4‐NP exposure was equal to that at which testis‐ova and sex reversal were induced, confirming that the gsdf gene in O. latipes embryos is a useful biomarker for predicting EDCs acting on gonadal sexual differentiation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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