| Autor: |
Basyoni, Hoda Ahmed, Mohamed, Karima Mukhtar, El-Barrany, Usama Mohamed, Rashed, Laila Ahmed, Aboubakr, Heba Mohamed, Razik, Heba Abdo Abdel |
| Předmět: |
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| Zdroj: |
Indian Journal of Forensic Medicine & Toxicology; Oct-Dec2020, Vol. 14 Issue 4, p2941-2948, 8p |
| Abstrakt: |
Background: A key element of forensic genetics is the ability to remove DNA from semen stain. Using various laundering conditions to remove semen stain is a routinely faced forensic problem Objective: This study was conducted to evaluate the possibility of detection of semen DNA in different types of fabrics after being washed. Method: Semen samples were applied to cotton and polyester clothes. After drying, the clothes were submitted to washing in water at room temperature, in water at 50°C temperature and in detergent at room temperature. DNA extraction was done to estimate the quantity of extracted DNA by spectrophotometer. Results: Different DNA quantities still could be detected after washing. There was highly statistical significant decrease (P ≤ 0.001) in the amount of extracted DNA in all group samples (after washing) compared to control group. Washing by detergent proved to have the most destructive effect on the DNA stains on clothes followed by washing by water at 50°C while washing by water at room temperature showed the highest recovery of DNA in clothes. The amount of the DNA detected in cotton samples before washing was higher than the amount detected in polyester samples before washing. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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