| Autor: |
Fujita, Ryosuke, Inoue, Maki N., Takamatsu, Takumi, Arai, Hiroshi, Nishino, Mayu, Abe, Nobuhiko, Itokawa, Kentaro, Nakai, Madoka, Urayama, Syun-ichi, Chiba, Yuto, Amoa-Bosompem, Michael, Kunimi, Yasuhisa |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology; 1/20/2021, Vol. 11, pN.PAG-N.PAG, 10p |
| Abstrakt: |
Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima , oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoroshi viruses (OGVs). The three RNA-dependent RNA polymerase genes detected were phylogenetically related to Partitiviridae, a group of segmented double-stranded RNA viruses. Purified dsRNA from a late male-killing strain of H. magnanima revealed 24 segments, in addition to the RdRps, with consensus terminal sequences. These segments included the previously found male-killing agents MK1068 (herein OGV-related RNA16) and MK1241 (OGV-related RNA7) RNAs. Ultramicroscopic observation of purified virions, which induced late male-killing in the progeny of injected moths, showed sizes typical of Partitiviridae. Mathematical modeling showed the importance of late male-killing in facilitating horizontal transmission of OGVs in an H. magnanima population. This study is the first report on the isolation of partiti-like virus from insects, and one thought to be associated with late male-killing, although the viral genomic contents and combinations in each virus are still unknown. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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