| Autor: |
de Oliveira Lopes, Gabriel Augusto, Ferreira, Luciana Rabello, de Souza Trindade, Giliane, Fonseca, Antônio Augusto, dos Reis, Jenner Karlisson Pimenta |
| Předmět: |
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| Zdroj: |
Archives of Virology; 2021, Vol. 166 Issue 1, p243-247, 5p |
| Abstrakt: |
Pseudocowpox is a zoonosis caused by pseudocowpox virus (PCPV), which mainly affects cows but can be an occupational disease of humans. The aim of the study was to validate a quantitative polymerase chain reaction (qPCR) assay for the detection of PCPV. The assay was able to detect up to 1000 copies of PCPV per µL in field samples, with a sensitivity of 80% and a specificity of 100%. We did not observe any cross-reactivity between PCPV-positive samples and samples that were positive for other genetically similar viruses. The repeatability and reproducibility were adequate according to parameters preestablished in official test validation manuals. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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