| Autor: |
Duran, Peregrino G., Hufana-Duran, Danilda, Peralta, Matt Daniel B., Venturina, Emma V., Venturina, Hernando V., Parrish, John J. |
| Předmět: |
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| Zdroj: |
Philippine Journal of Veterinary & Animal Sciences; Jul-Dec2020, Vol. 46 Issue 2, p124-135, 12p |
| Abstrakt: |
Fourier harmonic analysis (FHA) assessed the sperm nuclear shape of 31 buffalo bulls with known fertility to identify the bull with top conception rates of 28-36% and lowest conception rates of 13-19%. The FHA model developed for water buffalo, identified harmonic amplitude 1 as critical in selecting optimum cutoff values based on discriminant analysis. Using the model, 44 other bulls were classified as High- (n=12) or Low-fertile (n=10), while the rest (n=22) were mid-fertile bulls. Five bulls from High- and Low-fertility groups were randomly selected for semen quality tests. The tests included fertilization of buffalo oocytes, sperm motility parameters and Hypo-osmotic swelling (HOS) of the plasma membrane integrity. Results showed higher cleavage and blastocysts development with significant difference of hatching rates (P=0.021) for bulls that were predicted to be of High- vs. Low-fertility. Progressive motility (67.2±2.9 vs. 51.9±2.9%), straightness (85.4±0.7 vs. 81.3±0.7) and linearity (53.3±0.8 vs. 49.8±0.8) were higher in High- than Low-fertility bulls (P<0.05). Further, HOS test showed that High-fertility bulls had higher sperm cells (68.0 ± 6.2) with functional integrity than Low-fertility bulls (59.1 ± 6.2) though difference was not significant (P=0.346). The results suggest FHA is a potential tool in predicting bull fertility in water buffalo bulls. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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