| Autor: |
El-Gebaly, Fathy E. |
| Předmět: |
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| Zdroj: |
Egyptian Journal of Experimental Biology (Botany); 2020, Vol. 16 Issue 2, p253-260, 8p |
| Abstrakt: |
Barley stripe mosaic virus (BSMV) works as a vector to deliver genes to plants through the Virus-induced gene silencing (VIGS) mechanisms. The VIGS is derived from the plant defense mechanism as a tool to understand the gene function and the expected phenotype in a short time. Conversely, The BSMV enables heterologous protein overexpression in plants through the virus-mediated overexpression (VOX) mechanism. This study aimed to compare the microprojectile bombardment by biolistic particle bombardment device (gene gun) with the agro-infiltration methods to deliver the BSMV with green fluorescent protein (GFP) and phytoene desaturase (PDS) genes to Brachypodium distachyon. GFP and BdPDS genes worked as visual markers to confirm the conversion of pCass4-RZ-ND18 plasmid into the intact BSMV. The findings showed that the GFP signals appeared during agroinfiltration pathway while the BSMV during the microprojectile bombardment approach failed to express the foreign GFP gene. Furthermore, the knockdown of BdPDS gene phenotype appears as a photo-belched strips on the B. distachyon leaves after inoculation of the BSMV with agroinfiltration pathway. Conversely, B. distachyon leaves which are injected with the microprojectile bombardment showed no significant changes in the phenotype compared to the wild type. The BdPDS downregulation phenotype was confirmed by the qRT-PCR results which showed a highly significant decrease in the BdPDS expression level during the agro-infiltration approach only. Eventually, the agroinfiltration approach is much effective than the microprojectile bombardment approach although the former approach needs much time and many confirmation steps. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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