Effects of the Hydrogen Sulfide Donor GYY4137 and HSP70 Protein on the Activation of SH-SY5Y Cells by Lipopolysaccharide.

Autor:	Yurinskaya, M. M., Garbuz, D. G., Afanasiev, V. N., Evgen'ev, M. B., Vinokurov, M. G.
Předmět:	HYDROGEN sulfide HSP70 heat-shock proteins REACTIVE oxygen species TUBULINS INFLAMMATORY mediators RECOMBINANT proteins ENDOCYTOSIS
Zdroj:	Molecular Biology; Nov2020, Vol. 54 Issue 6, p894-903, 10p
Abstrakt:	The effects of exogenous recombinant human heat shock protein Hsp70 and hydrogen sulfide donor GYY4137 on the mechanisms of endocytosis of lipopolysaccharide (LPS) by human neuroblastoma cells SH-SY5Y were studied. Hsp70 and GYY4137 have been shown to significantly reduce LPS-induced production of inflammatory mediators by SH-SY5Y cells, including reactive oxygen species, nitric oxide, TNFα, IL-1β, and IL-6. Both the recombinant protein Hsp70 and the hydrogen sulfide donor GYY4137 exhibited significant protective effects; however, the combined use of these agents did not lead to a cumulative effect. It has been shown that pinocytosis, as well as clathrin-, caveolin-, tubulin- and receptor-dependent endocytosis were involved in protecting the cells by both the hydrogen sulfide donor and Hsp70 from LPS-induced production of reactive oxygen species and NO. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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