| Abstrakt: |
This work aims to evaluate thecytotoxicity, antioxidant (SOD), histological changes in the testicular and sperm abnormality in male rats post exposure to lead acetate and the possible protective role of casein, which reducing heavy metal toxicities has raised worldwide. Animals divided to four groups for 45 days. Every group (n=5). Group1 controlGroup. treated with distal water. Group II, received 300mg/kg lead acetate for 45 days. Group III, received (300mg/kg) lead acetate + casein to 45days. Group IV, received casein 20mg/kg for 3 week and (300mg/kg b. wt./day) lead acetate with for 4 weeks. Cytotoxicity, antioxidant test, histological study on testis changes and sperm abnormally were estimated. The result showed clearly impact lead acetate on chromosomal aberration, antioxidant SOD, testicular tissue and sperm abnormality of male rats and degenerative changes in seminiferous tubules, vascular congestion, also depletion of the spermatogonia cells (some seminiferous tubules appeared with one or two layers of spermatogonia) for 45 days while and some abnormal sperm shape, the groups receiving casein after exposure for lead acetate were ameliorated the damaging effects. The lead causes a change in cytotoxicity ,physiological, histological; casein have ability to modifying physiological, histological effect induced by lead, the casein is good antioxidant compound and its mechanism of action based on metabolism and the free radical inside cells. [ABSTRACT FROM AUTHOR] |
