| Abstrakt: |
Molluscum contagiosum Virus cause Pearl disease, is a self-limited infectious skin disease, prevalent in children, sexually active adults and individuals with immunocompromise. 75 lesion samples were collected in Diyala Province, in outpatient dermatology clinics, from 30 September to 20 October 2019 from patients with Molluscum Contagiosum Virus after diagnosis by the specialist doctor, and 25 samples as a control group collected from healthy. After genome extraction, specific primer was detected by conventional PCR then sequencing. The results of the study showed that the highest incidence of infection with Molluscum virus using Conventional PCR for lesion samples was 53.3% in the age group (1-10) years, and the lowest incidence was in the age group (51-60) at 4%. The results also showed that the infection rate in males and in females, approximately similar 50.7% and 49.3% in females. The results revealed an increase in the incidence of Molluscum contagiosum virus infection in the urban 66.6% compared to the rural, where it reached 33.3%. The results showed that molluscum virus lesions of were more pronounced in the upper body (face) by 96%, lower in the lower part of the body, and by 4%. The results of the PCR to detect 100 isolated that were 75 postive samples for patients with MCV and 25 negative samples as a control group by using a primer showed all bands corresponding to 167bp. The results of sequence analysis for primer with 167bp for MC002L gene were shown in 5 isolates that were divided into two groups according to similarity. It was found that the matched rate was 97% when comparing each of these groups with the global isolates in NCBI. It was detected a silent mutation. Two isolated samples were recorded in the NCBI in the accession number for the registered isolates LC520235, LC520236. [ABSTRACT FROM AUTHOR] |
