| Abstrakt: |
Objective To compare the results of HIV rapid detection reagent in saliva and blood in MSM in Shenzhen, and we clarify the role of saliva rapid detection in HIV screening in this population. Methods The exudate in saliva and blood samples of the same subjects in 393 MSM in Shenzhen were collected, and the HIV immunoblotting kit was used as the reference reagent to confirm the consistency of salivary antibody detection kit (colloidal gold method) and human immunodeficiency virus (HIV) type 1+2 antibody detection kit (colloidal selenium method). Results Totally 393 MSM were recruited. The true positive, false positive, true negative, false negative cases for saliva test reagents were 120, 0, 272, 1, respectively. Those for blood test reagents were 121, 3, 269, 0, respectively. The detection results of the HIV western blot kit were taken as the gold standard to compare and analyze the detection results of saliva and blood detection reagent. The sensitivity, specificity, false-positive rate, false-negative rate and accuracy of exudate in saliva test reagents were 99.2%, 100.0%, 0.0%, 0.8% and 99.8%, respectively. Those of blood test reagents were 100.0%, 98.9%, 1.10%, 0.0% and 99.2%, respectively. Kappa test analysis showed that the results of rapid saliva and blood test were consistent with HIV diagnosis, and the Kappa values were 0.994 and 0.982, respectively. McNemar paired chi-square test was used to analyze the results of saliva and blood rapid test, and the difference was not statistically significant (P=0.125). Conclusion Saliva and blood screening results have a good consistency, exudate in saliva detection is noninvasive and fast, this method is worth promoting for HIV self-screening in high risk population. [ABSTRACT FROM AUTHOR] |
