| Autor: |
Sun, Weiming, Liu, Lanhua, Memon, Abdul Ghaffar, Zhou, Xiaohong, Zhao, Hongwei |
| Předmět: |
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| Zdroj: |
Biosensors (2079-6374); Dec2020, Vol. 10 Issue 12, p191, 1p |
| Abstrakt: |
Carbofuran (CBF) is an efficient and broad-spectrum insecticide. As testing indicators for water quality and agricultural products, CBF and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are regulated by many countries. The detection of CBF and 3-OH-CBF is of great importance for the environment and human health. However, an immunosensor detection method for the simultaneous analysis of CBF and 3-OH-CBF remains unavailable. Herein, we report a waveguide-based fluorescent immunosensor for detecting CBF and 3-OH-CBF, synchronously. The immunosensor is based on a broad-spectrum monoclonal antibody with high binding affinity against CBF and 3-OH-CBF. The linear detection ranges for CBF and 3-OH-CBF are 0.29–2.69 and 0.12–4.59 μg/L, with limits of detection of 0.13 μg/L for CBF and 0.06 μg/L for 3-OH-CBF, respectively. The whole detection process for each cycle is approximately 30 min. The results show a good application prospect for the rapid detection of CBF and 3-OH-CBF in water or agricultural products. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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