| Autor: |
Slonova, Darya, Posvyatenko, Alexandra, Kibardin, Alexey, Sysolyatina, Elena, Lyssuk, Elena, Ermolaeva, Svetlana, Obydennyi, Sergei, Gnuchev, Nikolay, Georgiev, Georgii, Severinov, Konstantin, Larin, Sergey |
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| Zdroj: |
Frontiers in Cellular & Infection Microbiology; 12/23/2020, Vol. 10, pN.PAG-N.PAG, 11p |
| Abstrakt: |
PGLYRP1/Tag-7/PGRP-S is one of mammalian peptidoglycan recognition proteins (PGRPs). Here, we demonstrate that human recombinant PGLYRP1/Tag-7/PGRP-S potentiates the response of murine macrophage-like ANA-1 cells and human macrophages to facultative intracellular pathogen Listeria monocytogenes. PGLYRP1/Tag-7/PGRP-S binds to the surface of L. monocytogenes and other bacterial cells but has no effect on their growth in culture. While PGLYRP1/Tag-7/PGRP-S treatment modestly enhanced phagocytosis of bacteria by ANA-1 cells, the intracellular survival of PGLYRP1/Tag-7/PGRP-S treated L. monocytogenes was strongly inhibited 2 h after internalization. PGLYRP1/Tag-7/PGRP-S treatment of bacteria boosted oxidative burst induction and increased the level of proinflammatory cytokine IL-6 produced by ANA-1, however, these effects happened too late to be responsible for decreased intracellular survival of bacteria. Our results thus suggest that PGLYRP1/Tag-7/PGRP-S acts as a molecular sensor for detection of L. monocytogenes infection of mammalian cells that leads to increased killing through a mechanism(s) that remains to be defined. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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