| Abstrakt: |
This study was conducted to investigate the influence of adding aqueous extracts of Urtica dioica (AEUD) and date palm pollen powder (AEDPP) to Tris extender on some post-cryopreserved semen characteristics of Holstein bulls for different preservation periods (cooling at 5°C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC). Seven Holstein bulls of 2.5-3 years old were used in the current study during the period from 20th November, 2017 to 20th August, 2018. Pooled semen was equally divided into three groups within one experiment. AEUD (0.01 g/50 ml extender) and AEDPP (0.02 g/50 ml extender) were added to Tris extender and comparisons in response were made with the control group (Tris extender, C). The AEUD and AEDPP groups exhibited greater (P<0.01) sperm's cell individual motility percentage as compared with the C group at cooling as well as 1, 2 and 3 months PC periods. Concomitantly, greater (P<0.01) live sperm percentage was observed in AEUD and AEDPP groups in comparison with the C group at all preservation periods. Lesser (Pd<0.01) abnormal sperm percentage were noticed for AEUD and AEDPP groups as compared with the C group at 48 hr., 1, 2 and, 3 months PC. Greater (P<0.01) acrosome integrity percentage was observed for AEUD as compared with the other two groups at 1, 2 and, 3 months PC. The AEUD and AEDPP groups exhibited greater (P<0.01) plasma membrane integrity percentage in comparison with the control group at all preservation periods. In conclusion, adding AEUD and AEDPP to Tris extender had a crucial role in improving some PC semen characteristics of Holstein bulls. [ABSTRACT FROM AUTHOR] |
