| Autor: |
Litvinova, L. S., Malashchenko, V. V., Shunkin, E. O., Shupletsova, V. V., Khaziakhmatova, O. G., Yurova, K. A., Melashchenko, E. S., Khlusov, I. A., Komarova, E. G., Chebodaeva, V. V., Sharkeev, Yu. P., Panin, Victor E, Fomin, Vasily M |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2020, Vol. 2310 Issue 1, p1-5, 5p |
| Abstrakt: |
Osteoimmunolgy describes the interactions between blood immune cells and human multipotent mesenchymal stromal cells (hMMSCs) as a basis of successful wound and fracture healing. The aim was to investigate in vitro interaction of adipose-derived hMMSCs and human blood mononuclear cells (hBMNCs) modulated by calcium phosphate (CaP) coating in 3D culture. Titanium plates (10 × 10 × 1 mm3) with micro-arc bilateral multilevel CaP coatings were used. Cell-IQ phase-contrast microscopy and the real-time cell analyzer (RTCA) showed the CaP coating and hBMNCs synergistic negative effects on hMMSC motility that could be conditioned by enhanced osteogenic differentiation of stromal cells. Indeed, a 10-fold increase in the bone mineralization around the CaP-coated samples was detected in the mixed (hBMNCs + hMMSCs) 3D culture. Thus, cellular and molecular crosstalk between hBMNCs and hMMSCs modulated by multilevel micro-arc CaP coating with bone-like topography is an effective 3D model to study in vitro the novel pathways of osteoimmunology. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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