| Abstrakt: |
Pure carbon sources, especially carbohydrates which are raw materials deriving from agro‐industrial processes, are often used for small‐scale single‐cell oil production by fermentation. The aim of this study was to investigate the effects of different pure carbon sources on cell growth, lipid accumulation, and γ‐linolenic acid (GLA) production by the filamentous fungus Mortierella isabellina DSM 1414 (Deutsche Sammlung von Mikroorganismen). The sugars utilized in this study are found extensively and abundantly in nature, especially in food raw materials and, in consequence, in agro‐food industry wastes or surpluses. Thus, the potential of many waste materials containing these sugars to be used in the production of single‐cell oil by fermentation could also be evaluated. The effects of the sugars utilized on cell growth, biomass production, and lipid production were investigated. Fatty acids were also analysed in the lipids produced at the end of the fermentations. Results showed that the maximum biomass production was 10.80 g/L in lactose‐based media, while the maximum oil production was 5.44 g/L in maltose‐based media. Oleic (20.42%–42.94%), palmitic (14.96%–22.19%), and stearic (9.00%–26.92%) acids were the major fatty acids along with linoleic acid (11.35%–18.67%) and GLA (3.56%–8.04%). The production of GLA as the target fatty acid was remarkable. This study indicates that agro‐industrial waste including most of the sugars utilized (except for arabinose and sucrose with lipid production of 0.81 and 0.28 g/L, respectively) can be employed for production of single‐cell oil by M. isabellina DSM 1414 which contains a high amount of GLA. [ABSTRACT FROM AUTHOR] |
Plný text