| Autor: |
Sullivan, Mark V, Stockburn, William J, Hawes, Philippa C, Mercer, Tim, Reddy, Subrayal M |
| Předmět: |
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| Zdroj: |
Nanotechnology; 2/26/2021, Vol. 32 Issue 9, p1-13, 13p |
| Abstrakt: |
We have developed a low-cost molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify myoglobin in a biological sample. The assay uses a previously unreported method for the development of microwave-assisted rapid synthesis of aldehyde functionalized magnetic nanoparticles, in just 20 min. The aldehyde functionalized nanoparticles have an average size of 7.5 nm ± 1.8 and saturation magnetizations of 31.8 emu g−1 with near-closed magnetization loops, confirming their superparamagnetic properties. We have subsequently shown that protein tethering was possible to the aldehyde particles, with 0.25 ± 0.013 mg of myoglobin adsorbed to 20 mg of the nanomaterial. Myoglobin-specific fluorescently tagged MIP (F-MIP) particles were synthesized and used within the assay to capture myoglobin from a test sample. Excess F-MIP was removed from the sample using protein functionalized magnetic nanoparticles (Mb-SPION), with the remaining sample analyzed using fluorescence spectroscopy. The obtained calibration plot of myoglobin showed a linear correlation ranging from 60 pg ml−1 to 6 mg ml−1 with the limit of detection of 60 pg ml−1. This method was successfully used to detect myoglobin in spiked fetal calf serum, with a recovery rate of more than 93%. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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