| Abstrakt: |
In shrimp, which rely largely on their innate immune system for defense against invading pathogens, the Toll‐pathway has been extensively studied, as it plays a crucial role in innate immune responses. Different innate immune genes in the Toll‐pathway of shrimp, Penaeus monodon viz., Toll‐Like receptor (PmToll), Myeloid differentiation factor‐88 (PmMyD88), and Tumor necrosis factor receptor associated factor‐6 (PmTRAF6) have been characterized and their role in immune response against white spot syndrome virus has been elucidated by real‐time PCR‐based expression analysis. In the current study, the role of these genes in the immune response against bacteria was investigated by determining the mRNA expressions of PmToll, PmMyD88, and PmTRAF6 in different tissues of P. monodon, upon experimental infection with Vibrio harveyi, using real‐time PCR. Seven tissues were selected, including hemocytes, lymphoid organ, gill, hepatopancreas, stomach, midgut, and hindgut. Significant upregulation of these genes was observed in hemocytes, lymphoid organ, and gut tissues at different time‐points after infection. Moreover, modulation of expression of the genes at different time‐points could be observed in primary cultured hemocytes in vitro upon exposure with pathogen‐mimicking ligands (lipopolysacchrides and peptidoglycans). These experimental results indicate that the Toll‐pathway plays an important role in the immune response against bacterial challenge. [ABSTRACT FROM AUTHOR] |
