| Autor: |
Boakari, Yatta Linhares, El-Sheikh Ali, Hossam, Dini, Pouya, Loux, Shavahn, Fernandes, Claudia Barbosa, Scoggin, Kirsten, Esteller-Vico, Alejandro, Lawrence, Laurie, Ball, Barry |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility & Development; 2020, Vol. 32 Issue 16, p1239-1249, 11p |
| Abstrakt: |
High blood urea nitrogen (BUN) in cows and ewes has a negative effect on embryo development; however, no comparable studies have been published in mares. The aims of the present study were to evaluate the effects of high BUN on blastocoele fluid, systemic progesterone and Day 14 equine embryos. When a follicle with a mean (±s.e.m.) diameter of 25 ± 3 mm was detected, mares were administered urea (0.4 g kg−1) with sweet feed and molasses (n = 9) or sweet feed and molasses alone (control; n = 10). Blood samples were collected every other day. Mares were subjected to AI and the day ovulation was detected was designated as Day 0. Embryos were collected on Day 14 (urea-treated, n = 5 embryos; control, n = 7 embryos). There was an increase in systemic BUN in the urea-treated group compared with control (P < 0.05), with no difference in progesterone concentrations. There were no differences between the two groups in embryo recovery or embryo size. Urea concentrations in the blastocoele fluid tended to be higher in the urea-treated mares, with a strong correlation with plasma BUN. However, there was no difference in the osmolality or pH of the blastocoele fluid between the two groups. Differentially expressed genes in Day 14 embryos from urea-treated mares analysed by RNA sequencing were involved in neurological development, urea transport, vascular remodelling and adhesion. In conclusion, oral urea treatment in mares increased BUN and induced transcriptome changes in Day 14 equine embryos of genes important in normal embryo development. Maternal high-protein diets or urea treatments have been shown to change gene expression and affect embryo development in bovine and human embryos. However, the effects of high blood urea nitrogen (BUN) on equine embryos are unknown. Oral urea administration to mares resulted in Day 14 embryos with higher urea nitrogen concentrations in the blastocoele fluid and transcriptome changes related to neurological development and urea transport. These findings improve our knowledge regarding the effects of high BUN on equine embryo development. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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