Determination of p53 biomarker using an electrochemical immunoassay based on layer-by-layer films with NiFe2O4 nanoparticles.

Autor: Ibáñez-Redín, Gisela, Joshi, Nirav, do Nascimento, Gustavo Freitas, Wilson, Deivy, Melendez, Matias E., Carvalho, André L., Reis, Rui Manuel, Gonçalves, Débora, Oliveira, Osvaldo N. Jr
Zdroj: Microchimica Acta; Nov2020, Vol. 187 Issue 11, p1-24, 24p
Abstrakt: A disposable electrochemical immunosensors is presented suitable to detect cancer biomarker p53 using screen-printed carbon electrodes modified with a layer-by-layer (LbL) matrix of carboxylated NiFe2O4 nanoparticles and polyethyleneimine, onto which anti-p53 antibodies were adsorbed. Under optimized conditions, the immunosensors exhibited high surface coverage and high concentration of immobilized antibodies, which allowed for detection of p53 in a wide dynamic range from 1.0 to 10 × 103 pg mL−1, with a limit of detection of 5.0 fg mL−1 at a working potential of 100 mV vs. Ag/AgCl. The immunosensors also exhibited good selectivity with negligible interference upon incubation in complex matrices containing high concentrations of proteins (i.e., fetal bovine serum and cell lysate). The immunosensor performance is among the best reported in the literature for determination of p53, with the additional advantage of being disposable and operating with low-volume solutions. Graphical abstract [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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