| Autor: |
Sosa, Froylan, Romo, Salvador, Kjelland, Michael E., Álvarez‐Gallardo, Horacio, Pérez‐Reynozo, Sandra, Urbán‐Duarte, David, De La Torre‐Sánchez, Jose Fernando |
| Předmět: |
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| Zdroj: |
Reproduction in Domestic Animals; Nov2020, Vol. 55 Issue 11, p1490-1500, 11p |
| Abstrakt: |
The pterostilbene (PT) molecule is a phytoalexin with a reducing effect on reactive oxygen species (ROS) and with a capacity to block lipogenesis. However, the potential reducing effects of PT on equatorial lipid accumulation and ROS have not yet been elucidated for in vitro‐derived bovine embryos. The present study evaluated the effects of concentrations of 3, 1, 0.33, 0.11 μM PT, and a vehicle group on the percentage of cleaved embryos, embryos with more than 6 cells, percentage of blastocyst on Day 7 and 8, percentage of transferable embryos on Day 7, the cell count and relative concentration of lipids. In the second experiment, the effects of 0.33 μM PT and a vehicle group within two different O2 environments (5% and 20%) were evaluated for ROS generation and the percentage of Day 8 blastocysts. In the first experiment, no significant differences were found between the treatments with PT and the vehicle group (p >.05) concerning the percentage of cleaved embryos and embryos with more than 6 cells. Lipid reduction was observed in the groups treated with PT versus the vehicle group (p <.05). The vehicle group showed a higher rate of blastocyst production on Days 7 and 8 (p <.05) and an increase in the percentage of transferable embryos on Day 7 compared to the PT treatment groups (p <.05). Cell counts were not significantly different between treatments with PT and the vehicle group (p >.05). In the second experiment, the O2 concentration did not significantly affect ROS generation (p >.05); however, the groups treated with PT (0.33 μM) had a reduction in ROS (p <.05). The O2 concentration also did not significantly affect the rate of blastocyst production on Day 8 (p =.7696). Future research should be conducted to ascertain whether the reduction of lipids could enhance the cryopreservation and post‐thaw viability of PT‐treated embryos. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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