| Autor: |
Azizah, Purwatiningsih, Wiyono, Hidayat Teguh, Muzakhar, Kahar, Purnama, Budi, Nugraha, Dewanta Arya, Anwar, Fuad |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2020, Vol. 2296 Issue 1, p1-7, 7p |
| Abstrakt: |
Hypothenemus hampei Ferr. is one of the most destruction pest in coffee berry. To control this pest by synthetic insecticide is harmful and damage for environment. A strategy to control H. hampei is by studying its life cycle. The egg stage until adult of H. hampei lives inside coffee beans and utilize polysaccharides of coffee beans as source of its metabolism. The result of investigation showed that in gut of H. hampei was found cellulolytic bacteria Isolate 10 (ISH 10) which is suspected as endosymbiont bacteria that helps in the digestive process and metabolism of H. hampei. Morphological characters of ISH10 colony are circular, flat elevation, edge of entire colony, and the cell shape is rod. ISH 10 was gram positive bacteria which gave positive results in catalase and negative for oxidase test. In addition, ISH 10 is able to ferment some sugars media test such as glucose, sucrose, fructose and mannitol. ISH10 produced positive reaction in simmon citrate and urease test, and ISH 10 unable to reduce nitrate. Based on results, ISH 10 similar to genus Brochothrix. ISH 10 can also produce cellulase with the highest activity of the crude enzyme 0,031 U/ml only in 72 hours of incubation at 30oC against 0,5% CMC in 20 mM acetate buffer pH 5. Further characterization of this cellulase is needed so that it can interfere the digestion of H. hampei through endosymbiont cellulolytic bacteria by using inhibitor or environmental factor of its cellulase enzyme. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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