Evaluation of three immunochromatographic tests for rapid detection of antibodies against SARS-CoV-2.

Autor: Guedez-López, Gladys Virginia, Alguacil-Guillén, Marina, González-Donapetry, Patricia, Bloise, Ivan, Tornero-Marin, Carolina, González-García, Juan, Mingorance, Jesus, García-Rodríguez, Julio, on behalf of the SARS-CoV-2 Working Group, Montero-Vega, María Dolores, Romero, María Pilar, García-Bujalance, Silvia, Cendejas-Bueno, Emilio, Ruiz-Carrascoso, Guillermo, Lázaro-Perona, Fernando, Falces-Romero, Iker, Gutiérrez-Arroyo, Almudena, Girón de Velasco-Sada, Patricia, Rico Nieto, Alicia, Loeches, Belén
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Zdroj: European Journal of Clinical Microbiology & Infectious Diseases; Dec2020, Vol. 39 Issue 12, p2289-2297, 9p
Abstrakt: Lateral flow immunoassays (LFIA) for rapid detection of specific antibodies (IgM and IgG) against SARS-CoV-2 in different human specimens have been developed in response to the pandemic. The aim of this study is to evaluate three immunocromathographic assays (Sienna®, Wondfo® and Prometheus®) for detection of antibodies against SARS-CoV-2 in serum samples, considering RT-qPCR as a reference. A total of 145 serum samples from 145 patients with clinical suspicion of COVID-19 were collected: all of the samples were tested with Sienna®, 117 with Wondfo® and 89 with Prometheus®. The overall results of sensitivity, specificity, positive predictive value and negative predictive value obtained were as follows: 64.4%, 75%, 85.5% and 47.8% with Sienna®; 45.2%, 81.8%, 80.5% and 47.4% with Wondfo® and 75.5%, 12.5%, 51.4% and 29.4% with Prometheus®. The accuracy of the test for Sienna®, Wondfo® and Prometheus® was 67.6%, 59% and 47.2%, with a prevalence of COVID-19 of 69.7%, 62.4% and 55.1% respectively. Sensitivity of the three tests (Sienna®, Wondfo® and Prometheus® respectively) along the three different stages was 36.6%, 18.8% and 68.6% in the early stage (first week); 81.3%, 74.1% and 90.9% in the intermediate stage (second week) and 100%, 83.3% and 100% in the late stage (third week). The results demonstrate that even though Prometheus® presented a high sensitivity, the specificity was notably lower than the other two tests. Sienna® showed the greatest contrast between sensitivity and specificity, achieving the best accuracy, followed by Wondfo®. The sensitivity of the three ICT assays was higher in late stages of the disease. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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