| Autor: |
Silva, Weberson P., Rocha, Raquel G., Felisbino, John K. R. P., Sousa, Raquel M. F., Munoz, Rodrigo A. A., Richter, Eduardo M. |
| Předmět: |
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| Zdroj: |
ChemElectroChem; 11/2/2020, Vol. 7 Issue 21, p4469-4476, 8p |
| Abstrakt: |
Tibolone (TIB) is a synthetic steroid banned by the World Anti‐Doping Agency (WADA) due to its similar properties to estrogen and progesterone. In this work, a simple and low‐cost electrochemical method for the determination of TIB and its metabolites (3‐α hydroxytibolone and 3‐β hydroxytibolone) in authentic human saliva is reported. Square‐wave voltammetry (SWV) with an unmodified boron‐doped diamond (BDD) electrode and phosphate buffer 0.1 mol L−1 were selected as the electrochemical technique, working electrode, and supporting electrolyte, respectively. Analytical characteristics of the proposed method include good repeatability (RSD <2.4 %), wide linear range (1 to 110 μmol L−1; r=0.999) and low detection limit (0.3 μmol L−1). Average recovery values (104±3 % for TIB and 98±5 % for the metabolites) for direct analysis of spiked saliva samples (only diluted in supporting electrolyte) indicate that no significant matrix interference was observed. The proposed method was also efficient for the determination of TIB in pharmaceutical samples (statistically similar results to those obtained by HPLC). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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