| Abstrakt: |
This study high lights on the anticoagulant, fibrino (geno) lytic and antiplatelet activities of Aqueous Extract of raw Tender Mango Seed (RGMS). RGMS showed varied molecular weight proteins bands from the range 65kDa to 14.3 kDa on SDS-PAGE under reduced and non-reduced condition in a similar way. When, RGMS was analyzed for its probable carbohydrate content using PAS-staining, only single protein at the low molecular weight region taken up the PAS stain. RGMS digested casein with the specific activity of 1.106 units/mg/min at 37°C. The proteolytic activity was inhibited by only 1, 10, Phenanthrolene but IAA, PMSF, EDTA and EGTA did not affect the activity, suggesting the presence of zinc dependent metalloprotease. Furthermore, RGMS showed strong anticoagulant effect in plasma coagulation time by extending the clotting time from control 250 sec to 1200sec. RGMS hydrolyzed only Aα chain of the fibrinogen without affecting Bβ and γ chains. In addition, RGMS degraded all the chains of fibrin clot in a dose dependent manner. Above all, RGMS also exhibited antiplatelet activity by inhibiting agonists ADP and epinephrine induced platelet aggregation of platelet-rich plasma. The platelet aggregation inhibition was an extent of 80% 96% respectively. Moreover, RGMS was non toxic to Red Blood Cells. Taken an account of said properties, RGMS could be further exploit in the treatment of thrombotic disorders. [ABSTRACT FROM AUTHOR] |
