| Autor: |
KEISUKE ADACHI, YUTA OHNO, KEITARO SATOH, AKIKO SHITARA, YASUNORI MURAMATHU, MASANORI KASHIMATA |
| Předmět: |
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| Zdroj: |
In Vivo; Nov/Dec2020, Vol. 34 Issue 6, p3271-3277, 7p |
| Abstrakt: |
Background/Aim: Cryopreservation of cell lines has been widely used in the laboratory; however, cryopreservation of organs is still considered to be difficult. The submandibular gland (SMG) of fetal mice is one of the best-characterized organs. We investigated the conditions for cryopreserving SMG rudiments. Materials and Methods: Embryonic day 13 SMG rudiments were cryopreserved with or without a cryoprotectant. They were thawed and incubated in DMEM/F12 medium. Moreover, the influence of EGF stimulation on the signaling cascade after frozen-thawing the rudiments was analyzed by Western blotting. Results: When SMG rudiments were cryopreserved without a cryoprotectant, all cells in the rudiments died. However, the SMG rudiments that had been preserved in a cryoprotectant showed branching morphogenesis. Additionally, the responsiveness of signaling cascades to EGF did not differ between frozen with a cryoprotectant and non-frozen rudiments. Conclusion: Cryopreservation might be a useful technology for preserving tissues from small organs, such as fetal SMG rudiments. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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