| Autor: |
Youssef, Fadia S., Ashour, Mohamed L., El‐Beshbishy, Hesham A., Ahmed Hamza, Alaaeldin, Singab, Abdel Nasser B., Wink, Michael |
| Předmět: |
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| Zdroj: |
Journal of Pharmacy & Pharmacology; Dec2020, Vol. 72 Issue 12, p1830-1839, 10p |
| Abstrakt: |
Objectives: This study aimed to explore the pharmacological properties of pinoresinol‐4‐O‐β‐D‐glucopyranoside (PG), isolated from prunes. Methods: In‐vitro antioxidant activity was assessed using ferric reducing antioxidant power (FRAP) and 2,2'‐azino‐bis [3‐ethylbenzothiazoline‐6‐sulfonic acid]‐diammonium salt (ABTS) assays. In‐vivo hepatoprotective activity was evaluated using CCl4‐induced hepatotoxicity mouse model. The antihyperglycaemic activity was determined in vitro using α‐glucosidase and α‐amylase inhibiting activity and in vivo using streptozotocin‐treated model. Molecular modelling was done on α‐amylase, α‐glucosidase, aldose reductase and peroxisome proliferator‐activated receptor gamma. Key findings: Pinoresinol‐4‐O‐β‐D‐glucopyranoside showed promising antioxidant activity in FRAP and ABTS assays with total antioxidant capacity equal 418.47 and 1091.3 µmol/g in terms of ascorbic acid, respectively. PG (50 mg/kg b.w.) exhibited a hepatoprotective activity in vivo as it lowered AST and ALT levels. PG showed a potent in‐vitro antihyperglycaemic activity as it inhibited α‐glucosidase with an IC50 value of 48.13 μg/ml. PG caused a prominent decline in serum glucose level by 37.83% in streptozotocin‐treated mice with promising elevation in insulin level of 25.37%. Oxidative stress markers were reduced by PG, and it showed a high fitting on α‐amylase and α‐glucosidase active sites. Conclusions: Pinoresinol‐4‐O‐β‐D‐glucopyranoside is a natural entity combating oxidative stress, hepatic damage and diabetes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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