Investigation of the Effect of a Single-Stranded Break on the Mechanical Parameters of DNA by Molecular Dynamics Method.

Autor: Volokh, O. I., Armeev, G. A., Trifonova, E. S., Sokolova, O. S.
Zdroj: Moscow University Biological Sciences Bulletin; Jul2020, Vol. 75 Issue 3, p136-141, 6p
Abstrakt: Early detection and repair of damaged DNA is important for cell functioning and survival. The recently proposed mechanism of intranucleosomal loop formation suggests the relaxation of DNA supercoiling accumulated during transcription through damaged chromatin. The degree of DNA relaxation is affected by the mechanical properties and structure of the double helix. In this work, the consequences from the introduction of a single-stranded break on the mechanical properties of a DNA fragment are studied using molecular dynamics. It is concluded that the introduction of a single-stranded break leads to decreased stiffness and higher elasticity of the damaged DNA molecule as compared to the intact one. This, in turn, may lead to relief in the supercoiling of the defective DNA and to the enzyme arrest. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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