| Autor: |
Tsyrulnyk, Andriy O., Andreieva, Yuliia A., Ruchala, Justyna, Fayura, Lyubov R., Dmytruk, Kostyantyn V., Fedorovych, Daria V., Sibirny, Andriy A. |
| Zdroj: |
Yeast; Sep2020, Vol. 37 Issue 9/10, p467-473, 7p |
| Abstrakt: |
Candida famata is a representative of a group of so‐called flavinogenic yeast species that overproduce riboflavin (vitamin B2) in response to iron limitation. Overproduced riboflavin accumulates in the cultural medium rather than in the cells suggesting existence of the special mechanisms involved in riboflavin excretion. The corresponding protein and gene have not been identified in yeasts. At the same time, the corresponding gene BCRP has been identified in mammal mammary glands. Several homologs of the mammal BCRP gene encoding putative riboflavin efflux protein (excretase) were identified in Debaryomyces hansenii. The closest homolog was expressed under the control of D. hanseniiTEF1 promoter in the riboflavin overproducing strain of C. famata. Resulted transformants overexpressed the corresponding gene and produced 1.4‐ to 1.8‐fold more riboflavin as compared with the parental strain. They also were characterized by overexpression of RIB1 and RIB6 genes of riboflavin synthesis and exhibited elevated specific activity of GTP‐cyclohydrolase II. Membrane localization of the riboflavin excretase was confirmed by fluorescent microscopy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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