| Abstrakt: |
In this study, we report on new developments, improved results and a new fabrication approach to a micro-electromagnet-based microfluidic device capable of capturing and releasing superparamagnetic beads and bead-bound MCF-7 cancer cells on a sensor site with high efficiency based on the challenges identified and reported in our preliminary studies (Gajasinghe et al. 2016). The device is biocompatible and fabricated by utilizing only traditional microfabrication methods. The microfluidic channel is coated with SU-8 to provide uniform and smooth channel surface for easy release of the captured viable cells without any chemical usage. We labelled MCF-7 cells with superparamagnetic beads via Ep-CAM antibody and observed capture rates of the superparamagnetic beads and MCF-7 cells are 99.9 ± 0.2%, and 94.4 ± 7%, and release efficiencies are 97.4 ± 2%, and 95.2 ± 3%, respectively. Furthermore, there was no significant degrade on viability was observed. Reusability of the device was also presented. Overall, our device is highly efficient, fast, low cost, and has potential be used for cell analysis, drug screening, and disease diagnostics. [ABSTRACT FROM AUTHOR] |
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