Protease Production by Streptomyces sp. Isolated from Brazilian Cerrado Soil: Optimization of Culture Medium Employing Statistical Experimental Design.

Autor: Luciana A. I. De Azeredo, Leda R. Castilho, Selma G. F. Leite, Rosalie R. R. Coelho, Denise M. G. Freire
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Zdroj: Applied Biochemistry & Biotechnology; 6/1/2003 12:00:00 AM, Vol. 108 Issue 1-3, p749-756, 8p
Abstrakt: Streptomyces are important microorganisms because of their capacity to produce numerous bioactive molecules. In the present work protease production, by Streptomyces sp. 594 isolated from a Brazilian Cerrado soil, was maximized by optimizing a low-cost culture medium composition (casitone and sugarcane molasses) using statistical experimental design. The final protease activity (56 U/mL) was 2.8-fold and 58-fold higher than that obtained in the beginning of this study, and in a previous work, using an actinomycete selection medium, respectively. Protease production, not growth associated, appeared to be modulated by an inducer system, whereby the C/N ratio seemed to play a significant role. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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