| Autor: |
Salah D. M. Hasan, Ricardo L. Zollner, Maria H. A. Santana |
| Předmět: |
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| Zdroj: |
Applied Biochemistry & Biotechnology; 4/15/2003 12:00:00 AM, Vol. 106 Issue 1-3, p403-412, 10p |
| Abstrakt: |
Allergenic extracts were produced from Drechslera (Helminthosporium) monoceras biomass cultured by solid-state fermentation using wheat bran as the substrate. The main fermentation variables were selected by statistical design, and the optimized biomass yield (1.43 mg/[g of dry substrate · d]) was obtained at pH 9.5 and 45.8% moisture. The allergenic extracts were produced from crude extract by protein precipitation and polyphenol removal. Proteins in the range of 16-160 kDa were identified in the extracts. Their reactions in patients were characterized by in vivo cutaneous tests (positive in 40% of the atopic patients) and by dot-blotting assays. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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