| Autor: |
Kadyan, Saurabh, Kumar, Naresh, Lawaniya, Ramakant, Sharma, Pradeep Kumar, Arora, Bhawna, Tehri, Nimisha |
| Předmět: |
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| Zdroj: |
Journal of Food Safety; Oct2020, Vol. 40 Issue 5, p1-11, 11p |
| Abstrakt: |
Current investigation was aimed to develop colorimetric tests for rapid detection of Escherichia coli/coliforms. These test (s) for E. coli and coliforms were developed using the modified E. coli selective medium (M‐ECSM) and coliform selective medium, respectively. The selective media contain a combination of group specific marker enzymes and selective agents. The marker enzymes were screened using chromogenic substrates wherein β‐D‐glucuronidase and glutamate decarboxylase were found specific for E. coli while β‐D‐galactosidase for coliforms. The selectivity of the media was achieved using different concentrations of ampicillin and gentamicin. The optimized test procedures enabled sensitive detection of 0.35 ± 0.10 log cfu/ml of E. coli and 0.57 ± 0.15 log cfu/ml of coliforms at 37°C within 14.30 ± 0.45 and 12.15 ± 0.30 hr, respectively. M‐ECSM selectively inhibited major Enterobacteriaceae contaminants (Salmonella, Shigella, and Yersinia) up to 6 log cfu/ml. Moreover, better selectivity of M‐ECSM was reported against tested commercial chromogenic media. Field evaluation of developed test (s) reported prevalence of E. coli/coliforms as 57.29/88.54% in 96 raw milk and 16.28/51.16% in 43 pasteurized milk samples. Further, test components were vacuum dried in the form of miniaturized point‐of‐need test for field application in dairy farms and industries with minimal infrastructural requirements. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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