| Autor: |
関村光太郎, 神田真軌, 林 洋, 松島陽子, 吉川聡一, 大場由実, 小池裕, 林もも香, 永野智恵子, 大塚健治, 橋本常生, 笹本剛生 |
| Zdroj: |
Food Hygienic & Safety Science / Shokuhin Eiseigaku Zasshi; 2020, Vol. 61 Issue 4, p109-118, 10p |
| Abstrakt: |
The determination of antibacterial agents for animals in swine muscles was improved by microbiological screening and liquid chromatography-mass spectrometry(LC-MS/MS)analyses. In the first instance, the residual drugs were extracted from the samples using the Na2EDTA-McIlvaine buffer(pH 6.0). Subsequently, the agents were purified utilizing a PLS-3 cartridge and extracted with acetonitrile. Considering the microbiological methods, the sensitivities of the investigated drugs were higher and the test plate conditions were improved using a new reference organism Geobacillus stearothermophilus. As a result, a microbiological screening approach able to detect 33 drugs at MRL was developed in Japan. Remarkable, no false positives were detected. Moreover, the same preparation method enabled rapid and reliable microbiological screening, resulting in efficient screening with no undeterminable results. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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