| Abstrakt: |
The reference diagnostic method of human abdominal Cystic Echinococcosis (CE) is imaging, particularly ultrasound, supported by serology when imaging is inconclusive. However, current diagnostic tools are neither optimal nor widely available. The availability of a test detecting circulating biomarkers would considerably improve CE diagnosis and cyst staging (active vs inactive), as well as treatments and follow-up of patients. Exosomes are extracellular vesicles involved in intercellular communication, including immune system responses, and are a recognized source of biomarkers. With the aim of identifying potential biomarkers, plasma pools from patients infected by active or inactive CE, as well as from control subjects, were processed to isolate exosomes for proteomic label-free quantitative analysis. Results were statistically processed and subjected to bioinformatics analysis to define distinct features associated with parasite viability. First, a few parasite proteins were identified that were specifically associated with either active or inactive CE, which represent potential biomarkers to be validated in further studies. Second, numerous identified proteins of human origin were common to active and inactive CE, confirming an overlap of several immune response pathways. However, a subset of human proteins specific to either active or inactive CE, and central in the respective protein-protein interaction networks, were identified. These include the Src family kinases Src and Lyn, and the immune-suppressive cytokine TGF-β in active CE, and Cdc42 in inactive CE. The Src and Lyn Kinases were confirmed as potential markers of active CE in totally independent plasma pools. In addition, insights were obtained on immune response profiles: largely consistent with previous evidence, our observations hint to a Th1/Th2/regulatory immune environment in patients with active CE and a Th1/inflammatory environment with a component of the wound healing response in the presence of inactive CE. Of note, our results were obtained for the first time from the analysis of samples obtained in vivo from a well-characterized, large cohort of human subjects. Author summary: Exosomes represent an important way of cell-cell communication. Their release into the bloodstream is similar to sending messages in a bottle that can safely reach target tissues and cells. In disease contexts, these vesicles provide a suitable source of markers of pathogenesis or carcinogenesis, as well as of the immune response and the host-pathogen interplay. In cystic echinococcosis (CE), the availability of a marker-based blood test would represent an extraordinary improvement for disease diagnosis, staging and follow-up. The active search for CE carriers, realized by ultrasound surveys of around 25,000 individuals in the HERACLES project, allowed the implementation of a proteomic biomarker discovery analysis based on plasma exosomes from CE patients. The identification of parasite proteins likely within host exosomes supports the occurrence of this important exchange of information through exosomes and suggests possible molecular markers to be further validated. Finally, our study provides complementary and supporting information to previous evidence on the types of response mounted by the immune system in the presence of active or inactive CE, exploiting data obtained in vivo, from a large, well-characterized cohort of subjects. [ABSTRACT FROM AUTHOR] |
