| Autor: |
Salehirozveh, Mostafa, Dehghani, Parisa, Zimmermann, Milan, Roy, Vellaisamy A. L., Heidari, Hadi |
| Zdroj: |
IEEE Sensors Journal; Nov2020, Vol. 230 Issue 21, p12488-12494, 7p |
| Abstrakt: |
The development of cost-efficient, sensitive and specific methods to detect amyloid-beta 42 ($\text{A}\beta 42$) biomarkers in cerebrospinal fluid and serum-samples is of considerable interest to enable early and reliable diagnosis of Alzheimer’s disease as a precondition for future disease-modifying therapies. This paper presents a reduced graphene oxide field effect transistor (r-GO FET) for label free ultrasensitive detection of an $\text{A}\beta 42$ -biomarker with RNA aptamer. The channel in the device was formed by reduction of graphene oxide nanosheets by self-assembly process. As a result, the interaction between $\text{A}\beta 42$ and RNA aptamer on the surface of r-GO channel caused a linear response in the shift of the gate voltage ($\text{V}_{\mathbf {TG}}$) where the minimum conductivity occurs. The r-GO FET can detect the biomarker in range of 1ng/ml to 1pg/ml at pH 7.4 with high specificity. The developed r-GO FET is a low-cost, highly sensitive and selective method for detecting tiny concentrations of $\text{A}\beta 42$ , which would also enable measurements in serum-samples. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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