| Autor: |
Komitopoulou, E., Bainton, N.J., Adams, M.R. |
| Předmět: |
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| Zdroj: |
Journal of Applied Microbiology; Nov2004, Vol. 97 Issue 5, p964-972, 9p |
| Abstrakt: |
e. komitopoulou, n.j. bainton and m.r. adams. 2004.To identify the role of oxidation–reduction (redox) potential in the premature growth inhibition and RpoS induction inSalmonellaserotype Typhimurium in competitive growth experiments.Oxidation–reduction potential was measured throughout the growth of a minority population ofSalm. Typhimurium in mixed cultures with other Gram-negative and Gram-positive organisms. Alux-based reporter was also used to evaluate RpoS activity inSalm. Typhimurium in competitor studies. In a mixed culture, the multiplication of a minority population ofSalm. Typhimurium was inhibited when competing Gram-negative organisms entered the stationary phase. This was not seen when the competing flora was Gram-positive. The change in redox potential during growth in mixed cultures was closely linked to the inhibition ofSalm. Typhimurium growth by Gram-negative competitors. An artificially induced drop in redox potential earlier during growth in mixed cultures with Gram-negative organisms reduced the time to RpoS induction inSalm. Typhimurium and thus inhibited its multiplication prematurely. In contrast, RpoS induction and growth inhibition were prevented under high redox potential conditions.This work shows that the inhibitory activity of competitive organisms can be mediated through their effect on redox potential-regulated RpoS induction.Redox potential is shown to be an important determinant ofSalm. Typhimurium growth, an observation with practical implications both for its control and detection. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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