Hypothermic effects on gas exchange performance of membrane oxygenator and blood coagulation during cardiopulmonary bypass in pigs.
Autor: | Akeho, Kazuhiro, Nakata, Hayato, Suehiro, Shoichi, Shimizu, Kouji, Imai, Kensuke, Yamaguchi, Akane, Matsumoto, Ken-ichi, Oda, Teiji |
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Předmět: |
ANIMAL experimentation
BLOOD coagulation BLOOD gases analysis CARDIOPULMONARY bypass CONFIDENCE intervals ENZYME-linked immunosorbent assay HYPOTHERMIA IMMUNOASSAY MEMBRANE oxygenators PULMONARY gas exchange SWINE THROMBIN PROTEOMICS QUANTITATIVE research DATA analysis software DESCRIPTIVE statistics MANN Whitney U Test DISEASE complications |
Zdroj: | Perfusion; Oct2020, Vol. 35 Issue 7, p687-696, 10p |
Abstrakt: | Introduction: Whether hypothermic cardiopulmonary bypass could attenuate both blood coagulation and platelet activation compared to normothermic cardiopulmonary bypass remains elusive. Methods: Biocompatibility of a polymer-coated cardiopulmonary bypass circuit was comparatively assessed by plasma proteomics between juvenile pigs undergoing hypothermic (23°C) cardiopulmonary bypass and those undergoing normothermic (37°C) cardiopulmonary bypass (n = 6, respectively). Plasma samples were taken three times: 5 minutes after initiation of cardiopulmonary bypass (T5, before cooling), just before declamping and rewarming (Tc), and just before termination of cardiopulmonary bypass (Trw, 120 minutes). Proteomic analysis was quantitively performed by isobaric tags for relative and absolute quantification labeling. Thrombin–antithrombin complexes (TAT III) were measured by enzyme immunoassay, and vitamin K–dependent protein C (PROC), β-thromboglobulin (TG), and P-selectin were measured by enzyme-linked immunosorbent assay. Blood gas analyses evaluated oxygenator performance. Results: Hypothermic cardiopulmonary bypass had a significantly higher PaO |
Databáze: | Complementary Index |
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