Regulation of Biocatalysis with the α-Chymotrypsin–Bowman–Birk Inhibitor Pair Immobilized on Magnetite-Gold Hybrid Nanoparticles Using a Non-Heating Low-Frequency Magnetic Field.

Autor: Veselov, M. M., Kolomoec, N. I., Blinova, A. R., Efremova, M. V., Chudosay, Yu. V., Prusov, A. N., Zhigachev, A. O., Golovin, Yu. I., Klyachko, N. L.
Zdroj: Moscow University Chemistry Bulletin; Jul2020, Vol. 75 Issue 4, p225-231, 7p
Abstrakt: Magnetite-gold hybrid magnetic nanoparticles of the dumbbell type were used to immobilize two proteins: α-chymotrypsin and the Bowman–Birk inhibitor. It is shown that under the influence of a low-frequency magnetic field (50 Hz), the activity of chymotrypsin bound in a complex with an inhibitor is drastically reduced depending on the magnetic field induction. We hypothesize that this effect is related to the aggregation of magnetic nanoparticles under the influence of a magnetic field. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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