| Autor: |
Singh, Kanchan B. M., Madhavan, Jayanthi, Sadhukhan, Raghunath, Chandra, Shivani, Rao, Uma, Mandal, Pranab Kumar |
| Zdroj: |
Horticulture, Environment & Biotechnology; Oct2020, Vol. 61 Issue 5, p929-937, 9p |
| Abstrakt: |
Tuberose (Polianthes tuberosa) cultivation is tremendously affected due to Meloidogyne incognita infection. Histological study of in vitro nematode infected tuberose roots showed that the root infection initiated within 2 days post inoculation (DPI) and root gall formation occurred at 6 DPI indicating established infection on the roots. The life cycle of M. incognita in tuberose roots completed within 45 DPI evident by the formation of large number of eggs. Our study established in vitro methods like shoot tip culture and callus mediated regeneration of tubers collected from nematode infection fields to obtain completely nematode free plantlets. Tubers of different varieties produced multiple shoot bud on Murashige and Skoog (MS) media containing 4 mg L−1 6-benzylaminopurine (BAP) and 0.1 mg L−1 α-naphthaleneacetic acid (NAA). Maximum numbers of plantlets were obtained for Calcutta Single (14.4 ± 2.0 per plant). Embryogenic callus was induced on MS medium supplemented with altered concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), NAA and BAP from leaf, flower, root and tuber explant. The maximum callus induction was obtained on MS containing 1 mg L−1 2,4-D, 1 mg L−1 NAA and 0.5 mg L−1 BAP (100%) and 1 mg L−1 2,4-D and 2.25 mg L−1 BAP (96.7%). Regeneration of tuber callus was achieved on MS with 0.5 mg L−1 Kinetin (KIN) within 3 weeks. Plantlets were rooted on ½ MS with 0.5 mg L−1 Indole-3-butyric acid for 25 days. The in vitro regeneration protocol developed can thus be used for producing disease free plantlets for mass propagation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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