| Autor: |
Jambhulkar, Y. T., Madan, N . V., Mate, P. C., Gokhale, Niharika |
| Předmět: |
|
| Zdroj: |
International Journal of Pharmacy & Life Sciences; Jul2020, Vol. 11 Issue 7, p6836-6841, 6p |
| Abstrakt: |
Three simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of Rabeprazole (RAB) and Ondansetron (OND) in a laboratory mixture. Simultaneous equation method (Method I) shows absorbance at 284.5 nm (λ1) and 307 nm (λ2) corresponding to the absorbance maxima of RAB and OND respectively. In absorbance ratio method (Method II) isobestic point is observed at 291 nm. Isobestic point 291 nm is considered as (λ1) and absorbance maxima of OND at 307 nm are considered as (λ2). In First order derivative zero crossing method (Method III) (λ1) 247nm (for RAB) and (λ2) 320.5 nm (for OND) was carried out. All dilutions were prepared in distilled water and methanol (50:50). Linearity range was observed in the concentration range of solution 2.5‐12.5 μg/ml for RAB and 4-12 μg/ml for OND. The methods were validated statistically and recovery study was performed to confirm the accuracy of both drugs. The developed methods are simple, economic, accurate, precise and reproducible. They can be adopted for routine quality control analysis of these drugs in pharmaceutical combined dosage form. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
